Green synthesis of coumarin-pyrazolone hybrids: In vitro anticancer and anti-inflammatory activities and their computational study on COX-2 enzyme

A series of novel quinoline-2-carboxamides 4(a – l) were synthesized using different 2-oxo-2 H -chromene-4-carbaldehyde and 3-methyl-1H-pyrazole-5(4 H )-one with excellent yield under eco-friendly condition. All the compounds were confirmed on the basis of their IR, 1 H NMR, 13 C NMR and mass analysis. Newly synthesized compounds were screened for their anti-inflammatory by using protein denaturation method and anticancer activities at NCI60, Anti-inflammatory results are found to be promising. Further, molecular docking studies were performed for all the synthesized compounds with the active site of COX-2 enzyme and results obtained are quite promising. Compounds 4e and 4h exhibited highest potency and selectivity for COX-2 inhibitory activity.