Synthesis of 2H,13C-Labelled 2′-Deoxynucleosides and Their Site Specific Incorporation into Oligo-DNA for Structural Studies via Relaxation Time Measurements

Abstract We have recently shown1 the usefulness of 2H, 13C-labelled 2′-deoxynu-cleoside building blocks for structural studies via relaxation time measurements. The synthesis of phosphoramidite blocks 11 and 12 for their site-specific incorporation (indicated by underlines) into the d5′(1C2G3 A 4 T 5 T 6 A 7 A 8 T 9C10G)2 3′ is briefly described for studying the T1 and T1[sgrave] relaxations of 2H and 13C at specific deuterated carbons in a large molecule.