Molecular Cloning and Characterization of a Putative Promoter Region of mPC-1 Gene Homologous to hPC-1

To identify the regulatory region that are responsible for the expression of mPC-1,we have isolated and characterized the mPC-1 gene promoter.Sequence analysis of the mPC-1 5'-flanking region and a series of truncated constructs were performed,which were transiently transfected into the prostate cancer cell lines and non-prostate cancer cell lines and analyzed through Dual-luciferase reporter assay system.The relative activity of mPC-1 gene promoter was by far higher than pGL3-control containing SV40 promoter and enhancer and p61-PSA containing hPSA 6 kb promoter in AR(androgen receptor,AR)-positive prostate cancer cell lines.The region from 599 bp to 449 bp of mPC-1 promoter might contain a negative regulatory element.The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines.The relative activity of mPC-1 1.1 kb 5'-flanking region was regulated by androgen.The results demonstrated that the 1.1 kb fragment of mPC-1 5'-flanking region was relatively strong and prostate cancer cell specific promoter region.The 1.1 kb promoter of mPC-1 gene might be well suited to prostate cancer gene therapy if the promoter was properly modified.