Effect of Vitamin E Supplementation on Oxidative Stress and Biochemical Parameters in Diabetic Dogs

Twelve diabetic dogs were randomly assigned to two treatment groups consisting of six animals each. The first group (control) consisted of diabetic dogs that were treated with insulin @ 1.0 IU/kg BW while the second group (treatment) of diabetic dogs was supplemented with vitamin E @ 20.0 mg/kg BW as total dose along with insulin @ 1.0 IU/kg BW. The experiment continued for 45d and blood samples were collected on 0, 15, 30 and 45d for the study of biochemical and oxidative parameters. Dogs from vitamin E treated group showed significant decrease in glycated haemoglobin and fructosamine levels in comparison to control, which indicated better glycemic control from vitamin E treatment. There was significant changes (P<0.05) in biochemical parameters evident between the control vs. vitamin E supplemented groups in terms of glycated haemoglobin, fructosamine, triglycerides, total cholesterol, alakaline phosphatase and creatinine. Similar variations were also recorded with respect to oxidative stress indices, namely, lipid peroxidation, reduced glutathione, catalase and superoxide dismutase. Based on the clinical investigation, it is concluded that vitamin E supplementation along with insulin treatment is more beneficial in management of diabetes in dogs.