Research on the effect of camptothecin and its derivatives on proliferation and apoptosis of liver cancer cell HepG2

Objective To explore the effect of camptothecin( CPT) and its derivatives 10-hydroxy camptothecin( HCPT),7-ethyl camptothecin( SN22),7-ethyl-10-hydroxy camptothecin( SN38) on inhibition of hepatoma cell line Hep G2 and induction of its apoptosis. Methods The vegetative Hep G2 cells of logarithmic phase w ere assigned as control group and experimental group. Four drugs w ere made to different concentrations,w hich w ere 0. 01,0. 1,1,10,100 μmol / L. Different concentrations of drug solutions w ere effected on Hep G2 cells in 48 h,then M TT method w as used to detect the proliferation of HepG2 cell and cell apoptosis w as analyzed by flow cytometry( FCM) using Anexin V-PI staining. Results The results by M TT method show ed four drugs had inhibitory effect on proliferation of HepG2 cells,moreover,the proliferation inhibition of HepG2 cells w as enhanced gradually w ith the increase of drug concentrations in a certain scope drug concentrations,and the proliferation inhibition w as dependent on drug concentration. The sequences of proliferation inhibition w ere SN38,HCPT,SN22,and CPT. With different concentrations of camptothecin and derivatives w ith HepG2 liver cancer cells,there w ere significant differences compared w ith control group,and as drug concentration changed,cell apoptosis also presents gradient change; structure modification of SN38 w as stronger than SN22,CPT and HCPT function by FCM using Anexin V-PI staining. Conclusion Camptothecin and its derivatives can inhibit HepG2 cells and induce cell apoptosis.