Antiretrovirally Active Drug Hypericin Binds the IIA Subdomain of Human Serum Albumin: Resonance Raman and Surface-Enhanced Raman Spectroscopy Study
1998
Resonance Raman and surface-enhanced Raman spectroscopy were employed to study the interaction of hypericin with human serum albumin. The identification of the binding place for hypericin as well as the model for albumin−hypericin complex are presented. In this model hypericin interacts with tryptophan placed in II A subdomain of albumin. This interaction reflects (i) a change of the hydrophobicity of the tryptophan environment, (ii) the formation of an H-bond between the carbonyl group of hypericin and N1−H group of tryptophan, leading to a protonated-like carbonyl in the drug, (iii) a decrease of the strength of H bonding at the N1−H site of tryptophan, and (iv) a change of the tryptophan side-chain conformation.
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