Solid-State CP/MAS P-31 NMR in Studies of the Aminophosphonic Acids and Esters in Marine Animals

For the first time the 3 1 P solid-state NMR CP/MAS technique was applied to determine the amount of C-P forms in the total phosphorus in sea anemones. Signals of phosphonates (ca 20 ppm) and phosphates (ca 0 ppm) are well resolved and the phosphonate/phosphate ratios were easily calculated. Sea anemones Anemonia sulcata from the Adriatic Sea contain 41-54% of phosphorus as phosphonates (P-C). 3 1 PNMR analysis of lipids was also performed.