烟草普通花叶病土壤带毒与传播的检测与分析 Detection and Analysis of Soil Toxicity and Transmission of Tobacco Mosaic Virus

用分子生物学的方法对湖南省桂阳县烟区TMV常发地田间土壤样品及对应烟株进行了TMV检测，同时进行了实验室试验。结果表明：35个土壤样品中，有25个检测出TMV，TMV检出率为71.43%；相对应的烟株，带毒土壤TMV的传播率为88.0%。实验室试验表明：带毒土壤TMV的传播率为83.33%。由此说明，田间土壤中烟草TMV带毒是烟株TMV发病的重要传播途径。 The method of molecular biology was used to detect the soil samples and corresponding tobacco plants in the TMV often occur in Guiyang county, Hunan province. Laboratory tests were also carried out. The results showed that the detection rate of TMV was 71.43% among 35 soil samples. The transmission rate of TMV with toxic soil was 88.0%. Laboratory test showed that the transmission rate of TMV with toxic soil was 83.33%. The result shows that in the tobacco field, soil TMV virus is an important route of transmission road of tobacco TMV.