A New Tetracyclic Diterpene from Jatropha curcas

Jatrophodione A (1), a new diterpene with four rings, together with nine known compounds, caniojane (2), jatropholone A (3), jatropholone B (4), jatrogrossidione (5), 2-epijatrogrossidione (6), heudelotinone (7), gossweilone (8), (3 alpha)-3-hydroxy-ent-pimara-8(14), 15-dien-12-one (9), and 12-hydroxy-13-methylpodocarpa-8,11,13-trien-3-one (10), was isolated from the aerial parts of Jatropha curcas. Compounds 5, 6, 9, and 10 were found for the first time in this plant. Their structures were established by spectroscopic analysis, including 2D-NMR spectroscopic techniques. Cytotoxicities of compounds 1, 2, 7, 8, and 9 were tested on the three cancer cell lines A549, Hela, and SMMC-7721. Results showed that 7 exhibited cytotoxicity against SMMC-7721 with an IC(50) value of 21.68 mu m, whereas 7 and 8 were active against A549 with the IC(50) values of 16.04 and 20.47 mu m, and against Hela with the IC(50) values of 10.67 and 22.83 mu m, respectively.