Culture of Hepatocytes Using Non-Woven PTFE Fabric Coated with Copolymer of Poly (Amino Acid) Urethane

In this study, rat primary hepatocytes were cultured on a non-woven polytetrafluoroethylene fabric coated with a copolymer of poly (amino acid) urethane (PAU) to examine the expression of their original functions. In the culture dish, ammonium metabolic activity of hepatocytes cultured using the fabric coated with PAU is higher than that using collagen-coated polystyrene. It is also indicated that it is possible for hepatocytes to maintain the functions for long-term culture in the circulatory culture equipment with the fabric coated with PAU.