Comammox bacterial abundance, activity, and contribution in agricultural rhizosphere soils

Abstract The newly identified complete ammonia oxidation (comammox), which is capable of oxidizing ammonia directly to nitrate, has complemented our knowledge of nitrification in the global nitrogen (N) cycle. However, understanding the contribution and ecological roles of comammox in complex soil environments is still in its infancy. Here, the community structure and function of comammox and the interactions with other ammonia oxidation processes in rhizosphere and non-rhizosphere soils of four different crop fields (maize, cotton, soybean, and millet) were investigated in summer and winter. The only identified comammox species Candidatus Nitrospira nitrificans was widely distributed in all sampled soils. Comammox bacterial abundance was lower than that of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). The measured comammox potential rate ranged from 0.01 ± 0.002 to 0.40 ± 0.02 mg N kg−1 d−1, contributing