Single‐Irradiation Simultaneous Dual‐Modal Bioimaging Using Nanostructure Scintillators as Single Contrast Agent

: The rising demand for clinical diagnosis tools has led to extensive research on multimodal bioimaging systems. Unlike single-modal detection, multimodal imaging not only can provide both function and structure information but also can address the issue of sensitivity, depth, and cost. Despite enormous efforts, conventional step-by-step procedures for obtaining multimodal imaging pose a significant constraint on their practical applications. In this work, X-rays as highly penetrating radiation is proposed as a single-irradiation resource, while lanthanide-based nanostructure scintillators are employed as the single contrast agent to attenuate and convert X-rays, achieving computer tomography (CT) and optical dual-modal imaging at the same time. In other words, CT and optical dual-modal imaging are simultaneously produced via single radiation combined with single contrast agent. The function and structure information of targeted tumors in a mouse model can be clearly provided with large penetration and high sensitivity, indicating that this strategy is a simple but promising route for multimodal imaging of molecular disease and preclinical applications.