Lipid Peroxide Levels and Total Peroxyl Radical-Trapping Ability in the Placenta of Women with Normal Pregnancy and Preeclampsia

Objective : Our purpose was to investigate lipid peroxide levels, total peroxyl radical-trapping antioxidative parameter (TRAP) values. and antioxidant vitamin levels in placental tissues of women with preeclampsia and to evaluate their roles in the pathophysiology of preeclampsia. Study Design: Samples of placental tissue homogenates were obtained from 23 normal and 18 preeclamptic women between 33 and 40 weeks gestation. TNF- was assayed by an enzyme-linked immunoassay. Lipid peroxide levels measured by thiobarbituric acid reaction. The TRAP values were measured by modified Wayners method. Ascorbic acid, uric acid. retinol. -tocopherol, and -tocopherol were measured by high performance liquid chromatography (HPLC) CoulArray detector. Results : TNF- levels in placental tissue homogenates of women with preeclampsia were significantly higher than that of women with preeclampsia (21.43.39 vs. 10.31.06 pg/ml, p0.57 vs. 4.9S0.37 nmol/mg protein, p0.02 vs. 0.310.03 mM. p21.0 vs. 333.332.S nmo1/ml, p