Characterisation of acid dyes in forensic fibre analysis by high-performance liquid chromatography using narrow-bore columns and diode array detection

Abstract A gradient elution high-performance liquid chromatographic (HPLC) system with a diode array detector and a short narrow-bore (40 ×1 mm I.D.) column has been used to characterise a number of acid dyes. The resolution and reproducibility of the HPLC system have been evaluated and the detection limits for various dyes have been estimated. Comparisons are made with current methods of fibre dyestuffs examination used in forensic science. The system has been applied to the analysis of dye extracted from single fibres. Using diode array detection, both chromatographic and spectral data can be produced in a single operation from casework sized samples.