A unified GenomeSpace recipe to identify essential genes and associated subnetworks from Genome-Scale CRISPR-Cas9 knockout screens

We present a unified GenomeSpace recipe that combines the results of a high throughput CRISPR genetic screen and a biological network to return a subnetwork that suggests a mechanistic explanation of the screen’s results. The explanatory subnetwork is found by network propagation, a popular systems biology approach.  We demonstrate our pipeline on an alpha toxin screen, revealing a subnetwork that is both highly interconnected and highly enriched for hits in the screen.