Effect of citrated calcium sulphate on osteoblasts proliferation and differentiation

Objective:To study the effects of Citrated Calcium Sulphate(CCaS) on osteoblasts function and explore the possibility of this biomaterial as bone graft substitutes.Methods:Using the method of in vitro cell culcure,the influences of CCaS on the osteoblasts functions,such as cell proliferation,ALP activity,osteocalcin production,were detected.Results:Osteoblasts cultured in DMEM containing 100% concentration CCaS solutions growed slightly slower,but their relatively growth rates(RGR) were superior to 90%.They had the same AgNOR number as in control group,and excreted much more ALP and osteocalcin compared to in control group.Conclusion:CCaS has no toxtic effects on osteoblasts and stimulates the proliferation and differentiation of osteoblasts.