Chemical constituents from roots of Andrographis paniculata

To investigate the chemical constituents of the roots of Andrographis paniculata,28 compounds were isolated and identified from the 80% ethanol extract.There are 20 flavonoids: 5,5'-dihydroxy-7,8,2'-trimetroxyflavone(1),5-hydroxy-7,8,2',6'-tetramethoxyflavone(2),5,3'-dihydroxy-7,8,4'-trimethoxyflavone(3),2'-hydroxy-5,7,8-trimethoxyflavone(4),5-hydroxy-7,8,2',3',4'-pentamethoxyflavone(6),wightin(7),5,2',6'-trihydroxy-7-methoxyflavone 2'-O-β-D-glucopyranoside(8),5,7,8,2'-tetramethoxyflavone(10),5-hydroxy-7,8-dimethoxyflavanone(11),5-hydroxy-7,8-dimethoxyflavone(12),5,2'-dihydroxy-7,8-dimethoxyflavone(13),5-hydroxy-7,8,2',5'-tetramethoxyflavone(14),5-hydroxy-7,8,2',3'-tetramethoxyflavone(15),5-hydroxy-7,8,2'-trimethoxyflavone(16),5,4'-dihydroxy-7,8,2',3'-tetramethoxyflavone(17),dihydroneobaicalein(18),andrographidine A(19),andrographidine B(20),andrographidine C(21) and 5,2'-dihydroxy-7,8-dimethoxyflavone 2'-O-β-D-glucopyranoside(22);three diterpenoids: andrograpanin(23),neoandrographolide(24) and androgra-pholide(25);two phenylpropanoids: trans-cinnamic acid(26) and 4-hydroxy-2-methoxycinnamaldehyde(5);and oleanolic acid(9),β-sitosterol(27) and β-daucosterol(28).Compound 1 is a new flavone,compound 4 is a new natural product,compounds 2,3 and 5 were isolated from the Androggraphis genus for the first time and com-pounds 6-9 were isolated from this plant for the first time.