Chromosome Location of the Male-sterility and Yellow Seedling Gene in Line 1066A of Foxtail Millet

Using foxtail millet (Setaria italica (L.) Beauv.) male_sterile line 1066A as female parent and Yugu 1 primary trisomic series (1-7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male_sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F_1 generation of trisomics 2-9 were obtained by crossing with a great many plants of 1066A. F_1 generation of trisomics was similar to their male parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3∶1 in F_2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14∶1 only in F_2 generation of trisomic 6 (χ2_ 0.05=0.012). The segregation ratio of green seedling to yellow seedling is 12∶1 only in F_2 generation of trisomic 7 (χ2_ 0.05=0.31), but in other cases, this ratio is 3∶1. The results indicated that the male_sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.