Open-source raspberry Pi-based operant box for translational behavioral testing in rodents.

BACKGROUND Rodents have been used for decades to probe neural circuits involved in behavior. Increasingly, attempts have been developed to standardize training paradigms across labs; and to use visual/auditory paradigms that can be also tested in humans. Commercially available systems are expensive and thus do not scale easily, and are not optimized for electrophysiology. NEW METHOD Using the rich open-source technology built around Raspberry Pi, we were able to develop an inexpensive (<$1000) visual-screen based operant chamber with electrophysiological and optogenetic compatibility. The chamber is operated within MATLAB/Simulink, a commonly used scientific programming language allowing for rapid customization. RESULTS Here, we describe and provide all relevant details needed to develop and produce these chambers, and show examples of behavior and electrophysiology data collected using these chambers. We also include all of the tools needed to allow readers to build and develop their own boxes (CAD models for 3d printing and laser-cutting; PCB-board design; all bill of materials for required parts and supplies, and some examples of Simulink models to operate the boxes). COMPARISON WITH EXISTING METHODS The new boxes are far more cost-effective than commercially available environments and allow for the combination of automated behavioral testing with electrophysiological read-outs with high temporal precision. CONCLUSION These open-source boxes can be used for labs interesting in developing high-throughput visual/auditory behavioral assays for ∼ 10th the cost of commercial systems.