Structure and activity of artificial mutant variants of human growth hormone

In this report we describe the construction of two types of variant. One variant is mutant at Trp86, which is the single Trp residue in hGH, and moreover, is conserved in all growth hormones known so far. The other type of variant has a deletion in the long loop region that connects helices I and II, according to the crystal structure. We prepared these variants of hGH by in vitro mutagenesis and studied their biological activities and physicochemical characteristics