Mutant prevention concentrations of antibacterial agents to ocular pathogenic bacteria

Objective To establish a method to measure mutant prevention concentration (MPC) in vitro, and to measure MPC of antibacterial agents for ocular bacteria caused keratitis. Methods It was an experimental study. Forty strains of ocular bacteria were separated from cornea in Beijing Institute of Ophthalmology,which included 8 strains of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Klebsiella pneumoniae respectively. The minimal inhibitory concentration (MIC) of the levofloxacin (LVF),ofloxacin (OFL),ciprofloxacin (CIP),norfloxacin (NFL), tobramycin (TOB) and chlormycetin (CHL) were determined by agar dilution method from National Committee of Clinical Laboratory Standard (NCCLS). The MPC were measured by accumulate-bacterial methods with bacterial population inoculated more than 1.2×1010 colony forming units per milliliter with Mueller-Hinton broth and tryptic soy agar plate. With the software of SPSS 11.0,the datum such as the range of MIC, MPC, MIC90 and MPC90 were calculated, and the selection index (MPC90/ MIC90)and mutant selection window (MSW) were obtained. Results The MIC90 of INF and TOB(4 mg/L)to Staphylococcus aureus strains were the lowest. CIP showed the lowest MIC90(0.25 mg/L)to Pseudomonas aeruginosa among six kinds of antibacterial agents. The MIC90 of LVF to Staphylococcus epidermidis (256 mg/L),Streptococcus pneumoniae (1 mg/L) and Klebsiella pneumoniae (0.25 mg/L) were lower than other antibacterial agents. The MPC90, MSW and the MPC90/MIC90 of levofloxacin showed lower values compared with other antibacterial medicines. From all the datum, the MIC90 of CHL was the highest and the activity was the weakest. Although the activity of LVF was higher to every kind of bacteria, CIP had the highest activity antibacterial to Pseudomonas aeruginosa. The capacity of CHL and TOB was weaker than Quinolones for restricting resistant mutants on ocular bacteria. LVF had the strongest capacity for restricting resistant mutants among Qulnolones. Conclusion LVF has better antibacterial effects and stronger capacity for restricting the selection of resistant mutants on ocular bacteria than other antibacterial agents. (Chin J Ophthalmol, 2009,45:61-65) Key words: Drug resistance,bacterial;  Microbial sensitivity tests;  Anti-bacterial agents