avidin-biotin-peroxidase complexes. Localization of biotinylated gonadotropin releasing hormone on pituitary monolayer cells with

2% of the mono-layers. Capping was evident by 3 mm after exposure andthe stain appeared in dense patches, vesicles, or granules10-30 mm after exposure. The stain was abolished by theaddition of a 10- to 100-fold excess of unlabeled [D-Lys#{176})GnRH. Biotinylated GnRH released luteinizing hormone(LH) and follicle stimulating hormone (FSH) and was eitherequipotent or 10 times more potent than the unlabeledRecent studies have described gonadotropin releasing hormone (GnRH)