A process for the purification of biomolecules

The invention relates to a process for the purification of biomolecules from a sample, comprising the steps of: a) placing a reaction vessel having a binding matrix in a centrifuge, is prepared before or after this step, a solution or suspension of a biomolecule-containing sample in the reaction vessel and introduced into the reaction vessel; such as b) placing at least one multi-stage centrifugation step, consisting of at least a first centrifugation step at a first acceleration value and at least a second centrifugation step at a second acceleration value, which is higher than the first acceleration value; in which c) it is in step b) can be a binding step, a washing step and / or an elution step.