Sample pretreatment method for detecting different clenbuterol residuals

The invention relates to a sample pretreatment method for detecting different clenbuterol residuals. In the method, no halogen preparation is used, liquid-liquid extraction processes after alkalization are reduced, and the operation process is simple and fast. The method adopts an enzymolysis, extraction and purification manner for sample pretreatment, particularly, a zymolytic sample is directly purified through an MCX solid phase extraction column after being extracted through acidified methyl alcohol, and detection is performed through liquid chromatography tandem mass spectrometry. Twenty four samples can be detected within 6 hours, so that the detecting time is saved by about half, and the detecting cost is also lowered greatly.