Photoautotrophic production of eicosapentaenoic acid.

Eicosapentaenoic acid (EPA) is an omega-3 fatty acid which is an essential nutrient for both humans and animals. This review examines the global need for EPA, both in human nutrition and aquaculture. The potential shortfall in supply of this important nutrient as well as sustainability issues with wild-caught fish have generated increased interest into alternative sources of EPA. Various approaches are summarized, including heterotrophic production and the use of genetically modified microorganisms and plants. Studies on photoautotrophic production of EPA are extensively reviewed. Widely used species for large-scale production of EPA includes Phaeodactylum tricornutum and Nannochloropsis due to their robustness and relatively high growth rates and EPA content (typically 5% of dry biomass). Approaches for large-scale production have also been reviewed. Closed reactors like flat panels, tubular reactors and bubble columns may be the most suitable due to their high productivity. However, there is no agreement in the literature as to which design generates the lowest cost of production. The economics of the process has also been examined. The best estimates for large-scale (100 hectare) plants give EPA prices of the order 39-90 USD per kilogram. This is approximately ten times higher than the price of EPA derived from fish oil. Potential avenues for lowering the cost are highlighted, along with the need to better understand the advantages and disadvantages of different EPA production methods from a more holistic perspective.