Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes

Abstract Objectives A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes. Design and methods A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The biosensor measures current when polarized at 0.4 V. Results The sensor showed optimum response within 2 s  at pH 7.0 and 25 °C. The current (mA) was in proportion to concentration of TG in the range 0.56–2.25 mM. The minimum detection limit of the method was 0.21 mM. The analytic recovery of added TG was 94.3%. Within batch and between batch coefficients of variations (CV) were r  =   0.99) was found. Among various serum substances tested, only cholesterol caused slight stimulation (20%). Conclusions An amperometric method was developed for determination of TG employing this enzyme electrode.