The Fabrication of Injectable Polyiactide Porous Microcarriers for the Culture of the Bone Marrow Stroma Cells

Objective To develop a microcarrier which the pores size and the porosity of the could be controlled by changing the concentration of the ammonium bicarbonate solution, and investigate the effects of preparation condition on the size and morphology of the microcarrier. Methods An injectable poly-D, L-lactic acid Porous scaffold microcarrier was fabricated by the method of modified water-in-oil-in-water (W1 / O / W2). The microcarriers were characterized by means of SEM and granularity analysis. Results SEM showed that the average diameter of the microspheres was 335μm±65μm and the diameter of the pores was larger than 20μm that was large enough for cell infiltration and seeding. The images of SEM testified that the microcarriers prepared using greater amount of ammonium bicarbonate had larger internal pore volume. The results of cell cultured showed that BMSC grown well on the surface and in inner. Conclusion These porous scaffold microcarriers could be used for delivering the seeded cells to the tissue defect site in an injectable manner.