Development of Experimental Oil based Inactivated HS Vaccine from field Isolates of Pasteurella multocida from Cattle in Bangladesh

The present research work was conducted to developed oil based inactivated HS vaccine from fields isolates of Pasteurella multocida from cattle in Bangladesh and determination of antibody titers following vaccination in bovines. For this purpose, a total of 26 samples were collected where 16 blood samples were from Bogra and 6 blood samples from Mymensingh in clinical cases and 4 morbid materials such as lung, liver, and heart were collected from suspected cases of hemorrhagic septicemia (HS) in cattle of Rajshahi. The samples were processed for demonstration of bipolar organisms and isolation of Pasteurella multocida. There were 9 samples were isolated as P. multocida in which 3 samples from Bogra, 2 samples from Mymensingh and 4 samples from Rajshahi. The isolates were identified by cultural and morphological characteristics, biochemical characteristics, serological and pathogenicity tests. The dense culture of the organism was achieved in a blood agar by incubation. The formalin inactivated HS vaccines (oil adjuvanated) was prepared and administered in cattle at the dose rate of 2ml (1.5 OD600 = ≥7.5×108 CFU/ml) through subcutaneous (SC) route in each selected Group (A, and B) in the field of Gouripur Upazilla, Mymensingh and Nokla Upazilla, Sherpur respectively. Blood was collected from each group A and B before and after 28 days of primary vaccination and pre-vaccinated sera was kept as control. The mean PHA titers were 134.86, 169.14 and <4±0.00 in Group A and B and control group respectively. The PHA titers were analyzed by t-test to determine the immune level of vaccinated cattle. The vaccine produced better immune response in post vaccinated groups of cattle as compared to prevaccinated groups of cattle. So, it may be concluded that oil based inactivated HS vaccine induced high level of passive haemagglutinating (PHA) antibodies in the vaccinated cattle.