BACTERIAL ISOLATION AND ANTIBIOTIC SENSITIVITY TEST FROM URINE OF BUFFALO CALVES (Bubalus bubalis) AFFECTED WITH URETHRAL OBSTRUCTION

The study was conducted in 31 buffalo calves suffering from obstructive urolithiasis to isolate the bacteria and antibiotic sensitivity tests from their urine. Urine of the buffalo calves were collected via Foley’s catheter or an infant feeding tube in a sterile test tube. Urine samples were subjected to culture isolation and antibiotic sensitivity tests. Out of 31 samples, 13 samples (41.94%) showed no growth up to 3 days of incubation period whereas the remaining 18 (58.06%) urine samples showed bacterial growth. The most commonly isolated organisms were Escherichia coli, Staphylococcus species and Proteus species. The cephalosporin and Fluoroquinolone groups of antibiotic were more sensitive against isolated organisms.