[Chromosome aberrations induced in the lymphocytes of rabbits by ultrasounds of 1MHz alone or followed by treatment with 5-bromo-2'-deoxyuridine].

Rabbit lymphocytes were treated in Go with therapeutical ultrasound waves (1 W/cm2; 1 MHz; continuous wave mode) alone or followed by an additional exposure to 5-bromo-2'-deoxyuridine (BrdU). Doses in the Fresnel zone ranged from 120 to 960 J/cm2; no refrigeration was provided, and temperature increased from 37 degrees C to 45 degrees C. In the Fraunhofer zone, only an ultrasound dose of 960 J/cm2 was used, and the temperature of the insonated blood sample was maintained at 37 degrees C. For the sequential treatment, BrdU (10 micrograms/ml medium) was added immediately after the ultrasound treatment and was left in contact with the lymphocytes for 48 hours. Ultrasound alone did not induce chromosome aberrations, but ultrasound followed by the BrdU exposure resulted in an increased number of abnormal cells.