Laboratory Evaluations of Factors Affecting Biodegradation of Sulfolane and Diisopropanolamine

Sulfolane and diisopropanolamine (DIPA) are used in the Sulfinol® process to remove hydrogen sulfide from sour natural gas. This process has been used in western Canada since the early 1960s, and contamination of groundwater has occurred from surface spills and from seepage from landfills and unlined process water storage ponds. Aquifer sediments from contaminated and uncontaminated areas, and muds in a wetland downgradient from the contaminated plume, were collected from a gas plant. Vigorously agitated shake-flask cultures and gently agitated 2.5-L microcosms consisting of contaminated sediment, mud and groundwater, or wetland water were used to study the biodegradation of sulfolane and DIPA. The aerobic shake-flask method showed that all five of these materials contained microbial communities that biodegraded both compounds. Microorganisms in all samples, except the uncontaminated aquifer sediment, degraded both compounds in the aerobic 2.5-L microcosms. In general, the biodegradation occurred more rap...