Application of Prickly Ash Seed Cust Oil on the Preparation of Fatty Acid Methyl Ester by Lipase Catalysis

In this research, results of an experimental study of prickly ash seed cust oil on the preparation of fatty acid methyl ester by lipase catalysis were presented. Factors such as n-hexane, the FAME rate, water concentration, volume of lipase, et al were studied. The experiment results showed that n-hexane is most suitable solvent in the non-aqueous lipase catalytic system and the FAME rate reach the optimum when n-hexane concentration is 15%(w/w), water play an important role in the system and 10% is optimal concentration, When amount of lipase was 25%(w/w), n-hexane concentration was 15%(w/w), water content was 10% (w/w), methanol: oil was 3:1, the conversion rate was as high as 82.5% with pre refined oil and the conversation rate reach 93.5% after refine the oil at optimum experimental condition.