Detection of polymorphism of FecG gene in indigenous sheep of Arunachal Pradesh

Various physiological processes having direct impact on litter bearing ability namely folliculogenesis, oogenesis and ovulation are modulated by FecG or growth differentiation factor 9 (GDF9) on chromosome 5. Therefore, a study on molecular analysis of FecG gene was done for a better understanding of the reproductive ability of the indigenous sheep of Arunachal Pradesh. The aim of the study was to identify allelic variants of ovine FecG. Blood samples were collected from 50 randomly selected ewes. Genomic DNA extracted and PCR amplified for FecG gene with specific primer set. PCR fragment with length 139 bp was obtained. Genotypes were determined by restriction fragment length polymorphism (RFLP) method with DdeI restriction enzyme. Upon sequencing, a single restriction site was found. Thus, producing a single genotype AB, made up of two segments of 100 and 39bp. The locus of FecG gene was found to be monomorphic. According to the results the investigated animals were non-carriers of the mutation.