Novel methodology for the determination of alkaline phosphatase isoenzymes in plasma circulating

dp er os+1.5μg/kg/w sc. Eight rats were sham operated. Histomorphometric and histopathological studies were performed on tibias obtained after 30 days of treatment. OVX decreased trabecular bone (%BV/TV), and Z prevented and raised it (Sham: 35.3±4.7, control: 21.5±1.5*, MFP: 17.1±1.0*, Z: 64.3±3.5*, MFP±Z: 52.2±3.2*, * p<0.05 vs sham, mean±SE, ANOVA). Histological examination showed, in the sham group, an adequate bone-hemopoietic (HT) tissue relationship and mature bone trabeculae (Tb). The control group showed higher HT, immature Tb, fewer osteoblasts (Ob) and higher osteoclasts (Oc) compared with the sham group. The MFP group showed abundant HT and thin Tb with abundant lining cells (LC) and Ob. In Z, anastomosed and immature Tb with invasive tendency on the marrow space were observed. A decrease in Ob and HT was also observed. In contrast, the MFP+Z group showed mature Tb with well defined marrow spaces. The trabecular edge was covered by LC and Ob. We conclude that Z treatment, alone or combined with MFP, prevented the bone loss caused by OVX. Its combination with MFP normalized the histological features of bone. The MFP, by stimulating osteoblast activity, could modulate bone remodeling by producing a better quality bone. This article is part of a Special Issue entitled AAOMM 2010 Abstracts.