Study on electrotransformation of vitro DNA into Pseudomonas putida.

Pseudomonas putida isolated relieving salt stress and promoting plant growth was used as the recipient strain.Optimum conditions including growth stage of the strain,concentration of competent cell,electroshock voltage and the meduim were investigated for the electroporation of P.putida-E.coli shulter vector PDSK519 into P.putida Rs-198.It was showed that the highest transformation efficiency was up to 1.3×107CFU/μ g DNA in the optimium condition,under which the competent cells were collected at logarithmic growth phase(OD600=0.5),the mixture of the competent cells(4.6×1012/ml)and PDSK519 plasmid DNA was electroporated at 13kV/cm.It will be very helpful for developing a genetic transformation system and studying the genomic function of P.putida.