Screening and application of fumonisin B1 aptamer strand displacement probe

2016 
The invention belongs to the biotechnological field, and relates to application and development of aptamers, in particular to screening and application of a fumonisin B1 aptamer strand displacement probe. Through screening of the FB1 aptamer strand displacement probe, a foundation is laid for research and development of a subsequent signal amplification sensor technique based on the FB1 aptamer strand displacement probe. An FB1 fluorescence polarization detection technique based on the strand displacement probe adopts an FB1 aptomer as a molecular recognition element and has the advantages of being low in cost and high in specificity compared with an immunodetection technique based on an antibody; in addition, the technique is a homogeneous-phase detection technique, the repeatability is good, operation is easy and convenient, parallel detection of a large quantity of samples can be achieved, building of an agricultural product fumonisin monitoring technique system suitable for the national conditions of China is facilitated, the urgent need of China for a key agricultural product fumonisin pollution monitoring technique is met, the important significance on ensuring the agricultural product consumption safety and life and health of people is achieved, and the application prospect is wide.
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