Heat-resisting cryoprotectant for classical swine fever live vaccines and preparation method and application thereof

The invention provides heat-resisting cryoprotectant for classical swine fever live vaccines and a preparation method and application thereof, and belongs to the technical field of veterinary biological product manufacturing. The cryoprotectant is prepared from, by mass, 0.2-2.5% of synanthrin, 0.5-12% of glucan, 1-6% of polyvinylpyrrolidone, 0.5-12% of lactose, 0.2-6% of saccharose, 0.05-2.5% of polyethylene glycol, 0.005-0.6% of histidine, 0.05-4% of cysteine and 0.2-4% of a DMEM culture medium, and the rest is water for injection. The invention further provides a preparation method and application of the heat-resisting cryoprotectant. The heat-resisting cryoprotectant does not contain foreign protein or gelatin, stress reactions and side effects on animals are avoided, the safety of the vaccines is greatly improved, the antigen activity can be efficiently protected in the freeze-drying and storing process, and the stability of the vaccines is improved.