CARACTERIZAÇÃO MOLECULAR DE ISOLADOS DE MYCOBACTERIUM BOVIS DO ESTADO DE SÃO PAULO BRASIL, UTILIZANDO A TÉCNICA DE SPOLIGOTYPING

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM THE STATE OF SAO PAULO, BY THE USE OF THE SPOLIGOTYPING TECHNIQUE. This goal of this research was to study, by Spoligotyping, 43 Mycobacterium bovis isolates, from cattle slaughtered in the State of Sao Paulo, typed in 12 different spoligotypes. Among these isolates 35 were classified in 6 spoligotypes known already, and 8 isolates in 6 new spoligotypes. Within those, 29 isolates corresponded to the SB0295, SB0121 and SB0140 spoligotypes. The SB0140 spoligotype is the major type found in Argentina and is also present in Paraguay and Uruguay. The SB0120 spoligotype is the most frequently observed type in France (26%) and has also benn in Belgium, China, Denmark, Iran, Japan, Portugal, Russia, South Africa, Sri Lanka and the Netherlands. The AR28 spoligotype has also benn found in Argentina and Brazil. SB0122 was only cited in the Netherlands. It is important to emphasize that among the new spoligotypes found in the present study, at the moment, there are 6 unique spoligotypes, 2 of them isolated as N7 spoligotype and 2 isolated as the N8 spoligotype. The results show that there are in Brazil spoligotypes that, up to this moment, seem to be shared between Brazil, Argentina and Europe, and therefore the molecular discrimination of the M. bovis isolates by the Spoligotyping constitutes a tool for surveillance systems in detecting focuses of bovine tuberculosis.