Determination of the cytotoxic activity of Vibrio cholerae

: Cytotoxic activities of broth culture supernatants of 39 V. cholerae strains 01 and non 01 were studied in L-929 cell cultures. The examined strains differed by the cytotoxic factor production, which factors were identified (with the use of anti-cytolysin serum) as cytolysin. The strains capable of choleric enterotoxin secretion did not produce cytolysin.