Polaribacter vadi sp. nov., isolated from a marine gastropod

A Gram-reaction-negative, non-motile, yellow-pigmented, NaCl-requiring bacterial strain, designated LPB0003T, was isolated from a marine gastropod, the granulated dogwhelk (Thais luteostoma). The complete genome sequence of strain LPB0003T (GenBank accession number CP017477), sized 3.81 Mb, had 3296 protein-coding genes. 16S rRNA gene sequence analysis indicated that strain LPB0003T belongs to the genus Polaribacter within the family Flavobacteriaceae with sequence similarities of 95.3–97.8 % to other species of the genus Polaribacter . The average nucleotide identities with closely related species were not higher than 80 %. The isoprenoid quinone (MK-6) and DNA G+C content (29.6 mol%) of strain LPB0003T fell within the expected ranges for the genus Polaribacter . The major polar lipids of the isolate were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, an unidentified glycolipid, and two unidentified lipids. The predominant cellular fatty acids were iso-C15 : 0, iso-C15  : 0 3-OH, C16 : 1 ω7c and/or C16 : 1 ω6c (summed feature 3), and C15 : 1 ω6c. Based on phylogenetic, genomic and phenotypic data presented in this study, strain LPB0003T should be classified as a novel species of the genus Polaribacter , for which the name Polaribacter vadi sp. nov. is proposed. The type strain is LPB0003T (=KACC 18704T=JCM 31217T).