HPLC Analysis and Blood–Brain Penetration of 20-Hydroxyecdysone Diacetonide

Blood-brain penetration of 20-hydroxyecdysone 2,3;20,22-diacetonide (20DA) has been scouted using chromatographic methods. In vivo experiments were performed by treating male Wistar rats intraperitoneally (i.p.) with a dose of 50 mg kg−1 20DA. Control experiment was done by using 20-hydroxyecdysone (20E). Definite brain penetration of 20DA was found by using high-performance liquid chromatography (HPLC), while 20E does not show this type of distribution.