Molecular Techniques Commonly Used in Hemato-oncology

Molecular studies in hematological malignancies are based on nucleic acid analysis. The choice of molecular methods depends on molecular changes that are expected. The techniques described in this chapter include: methods of tissue collection, DNA and RNA extraction, DNA and RNA storage recommendations, DNA and RNA amplification (PCR, reverse transcription and real time quantitative PCR), electrophoretic analysis of the length of amplified fragments, DNA sequencing, analysis of DNA methylation, and analysis of large genomic deletions.