Identification of (−)-Δ9-6a,10a-trans-tetrahydro-cannabinol and two of its metabolites in rats by use of combination gas chromatography-mass spectrometry and mass fragmentography

Abstract Two metabolites of (−)- Δ 9 -6 a ,10 a - trans tetrahydrocannabinol (Δ 9 THC), one of the psychotomimetic components of Cannabis sativa L, were found after this substance was injected intraperitoneally into rats. Extracts of rat urine, blood, bile and feces were analysed by gas chromatography, mass spectrometry and mass fragmentography. In urine and feces unchanged Δ 9 THC was found. After glucuronidase treatment of feces, cannabinol (CBN) and an equal amount of Δ 9 THC was also found. Dihydroxy-Δ 9 -THC was identified in urine and feces. Diacetyl-Δ 9 THC was isolated from bile.