BtpA and BtpB TIR filaments are not coincident with yeast tubulin.

Indirect immunofluorescence of YPH499 yeast cells expressing GFP-BtpA-TIR, GFP-BtpB-TIR, and their corresponding E234A mutant versions from pYES2 plasmid derivatives (green). Microtubules are stained using anti-tubulin antibody (red). Nuclei are labelled with DAPI (blue). Scale bars correspond to 5 μm. (PDF)