Jaboticaba (Plinia peruviana) extract nanoemulsions: development, stability, and in vitro antioxidant activity

AbstractObjective: The aim of this work is to develop and characterize nanoemulsions containing jaboticaba extract (Plinia peruviana) aiming pharmaceutical and cosmetic applications.Methods: Nanoemulsions were prepared by high-pressure homogenization method using different concentrations of components (oil, surfactant, and extract) and homogenization pressures, in order to optimize the preparation conditions. Both unloaded and extract-loaded nanoemulsions were characterized according to their size, polydispersity, zeta potential, pH, morphology, and physical stability. Total phenolic and flavonoid contents in free jaboticaba extract and jaboticaba-loaded nanoemulsions were determined spectrophotometrically, while ellagic acid content was determined by high-performance liquid chromatography (HPLC) analysis. In vitro antioxidant activity was investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods.Results: Colloidal dispersions exhibited a mean particle size...