Radioimmunoassay Data Processing with a Small Programmable Calculator

Abstract We have developed three programs for data processing for radioimmunoassays RIA implemented on a small, inexpensive, programmable calculator (Texas Instrument Co., Model 59): 1) The first program performs a weighted logit-log regression for analysis of the RIA standard curve, and provides several descriptive statistics: slope, intercept, ED50 (and their corresponding standard errors), residual variance, and correlation coefficient. This program also provides: dose interpolation for unknowns; an estimate of the precision (percent coefficient of variation) for each result; corrections for variable sample volumes or recoveries; and the mean and standard error of the mean for samples analyzed in replicate. 2) The second program uses the “four parameter Logistic model” to describe the dose response curve and perform dose interpolation. This is the equivalent to the use of the Logit-log method, with provision for adjustment for the position of the 0% and 100% response. As such, it is more flexible and v...