Propagación in vitro de Hylocereus purpusii Britton & Rose, una especie mexicana en peligro de extinción

The purpose ofthis workwasto evaluate different conditions and culture parametersforthe in vitro establishment and multiplicaron of Hylocereus purpusii. Seeds were used as plant material and a workflowwas developed as an alternative forthe propagation and recovery ofthis species in danger of extinction. ln the establishment phase, the best result was obtained in the treatment with 1% NaOCI for 15 minutes achieving a 90% of germinated seeds and a final 77.7% of in vitro established plants. ln the multiplicaron phase when combining 8.88 uM 6-BAP with different concentrations of IAA, a significant increment in the length of the shoots was observedwith respecttothe control (no addition of IAA). Acombination of8.88 uM 6-BAP with 3.42 uM IAA was selected as the best multiplication condition reaching a multiplicaron rate of 8.8. ln the acclimatization phase, after two months of culture in ex vitro conditions the survival was higher than 98% and after six months of growth, the plants were transplanted to field. Key words: cactus; disinfection; growth regulators; seed germination