The effects of aging on the function of alveolar macrophages in mice.

In order to determine whether the function of alveolar macrophages (AM) is modulated by aging, we measured the TNF-α production, phagocytic function, and surface antigen expression of AM from young and old mice. When AM were primed by IFN-γ (500 units/ml) and triggered by LPS (100 μg/ml), TNF-α production by AM was significantly smaller in old mice as cmpared with young mice (young mice: 161.7 ± 28.2 units/ml; old mice: 89.3 ± 13.6 units/mlf, P < 0.05). The percentage of AM which phagocytosed latex particles (more than one particle) in old mice was significantly lower than in young mice (young: 78.1 ± 2.5%; old: 62.8 ± 3.4%, P < 0.05). Ia antigen expression of the AM was significantly higher and asialo-GM1 antigen expression was significantly lower in old mice than in young mice (Ia: young, 0.030 ± 0.005; old, 0.092 ± 0.024, P < 0.05; asialo-GM1: young, 0.97 ± 0.01; old, 0.75 ± 0.07, (P < 0.01). These results suggest that alveolar macrophage function is at least decreased in part with aging in mice.