Nanostructured Au-graphene modified electrode for electrosensing of chlorzoxazone and its biomedical applications

Abstarct The electrosensing of chlorzoxazone (CHZ) was studied for the first time ever at nanostructured Au/graphene modified glassy carbon electrode (AuNPs/Gr/GCE) employing different voltammetric techniques viz. cyclic, linear sweep and differential pulse voltammetry. The results indicated that the voltammetric response of CHZ was improved distinctly at the AuNPs/Gr/GCE electrode than at bare GCE. In order to carry out the CHZ determination, the dependency of the response current on supporting electrolyte pH, sweep rate and CHZ concentration were inspected to optimize the investigational conditions. The surface characterization of the Au/graphene modified and unmodified glassy carbon electrodes was carried out. The anodic peak showed a process of diffusion-control. The number of electrons transferred was calculated and was found to be two. The determination of [CHZ] using the said modified electrode was studied over the range of 1.0 × 10-7 M to 10.0 × 10-7 M which showed the LOD value to be 1.164 × 10-8 M. The study of excipients showed that the method owns selectivity. A differential pulse voltammetry which is fast and simple, showed a good accuracy and precision, for the purpose of determining the [CHZ] in biomedical applications like pharmaceutical formulation and also in pathological sample testing. In addition, this type of work can find its usage towards scientific purposes that involves electrical measurements, particularly in industrial quality management, environmental pollution management, as this involves simple sample preparation, appreciable repeatability, reproducibility and also high sensitivity.