Phytic Acid Anaylsis By Different Bacterial Phytases

This study aimed to measure the in vivo (using 70 male Cobb chicks experiments) degradation of the inositol hexaphosphate (IP6) to lower insitol phosphate (IP2-IP5) using different phytases from Aspergillus, Bacillus, E. coli and Klebsiella. The broiler chicks experiment was designed according to the GFE (1999) including two basal diets, one formulated to contain adequate levels of all nutrients (diet A-positive control) while the other was deficient in the phosphorus supply (diet B- negative control) supplemented with the above mentioned enzymes. The inositol phosphates were analyzed in faeces and ileum content of all broiler chicks groups, the results revealed lower concentration of IP2-IP5 and higher concentration of IP6 for all groups. Moreover, the inositol hexaphosphate (IP6) degradation was determined, on the basis of daily intake and excretion of inositol hexaphosphate (IP6). The IP6 degradation was significantly increased by the supplementation of different phytases. The highest degradation rate was 8.5 ± 3.7 µM/g dry matter in faeces caused by the supplementation of E. coli and 7.8 ± 3.5 µM/g dry matter in ileum by Bacillus phytases.